Custom DNA Oligos

We streamline primer selection for our customers by meticulously evaluating products based on essential criteria: purification technique, application suitability, synthesis duration, affordability, and further specifications, guaranteeing the best match for their unique research requirements.

  • Conventional primers

    Length: 10-60 bases

    Purification: Desalt, HPLC, PAGE

    Quantity: 2/5/10/50/100 OD

  • Long chain primers 1

    Length: 61-80 bases

    Purification: Desalt, PAGE

    Quantity: 1/2/5 OD

  • Long chain primers 2

    Length: 81-100 bases

    Purification: PAGE

    Quantity: 1 OD

Purification Options

Desalt

  • Applicable length: 10-90 bases
  • Application(s): PCR, Sanger sequencing, Gene synthesis; Meets most molecular biology laboratory needs.

PAGE

  • Applicable length: 10-100 bases
  • Application(s): PCR, Sanger sequencing, Gene synthesis, mutant subclone, gene construct, Better for long chain primer purification.

HPLC

  • Applicable length: ~60 bases
  • Application(s): PCR, Sanger sequencing, Gene synthesis, mutant subclone, gene construct, Antisense, modified primer; Application to purification of primers and probes for commercial diagnostic kits.

PAGE+HPLC

  • Applicable length: 10-100 bases
  • Application(s): PCR, Sanger sequencing, Gene synthesis, mutant subclone, gene construct, Antisense, modified primer; Suitable for long fragments and products that require increased purity

NOTE:
1. Desalt, Oligos are processed through normal-phase chromatography column, which removes salts but not failure sequences;
2. PAGE, Polyacrylamide gel electrophoresis (PAGE) is a method used to differentiate full-length product from failure sequences based on size and conformadtion.
This method removes short-stranded primers from the synthesis and works very well for long-stranded primers (>60 bases).
3. HPLC, Reverse-phase high-performance liquid chromatography (HPLC) removes failure sequences or unincorporated labels the same way as cartridge purification. The method achieves high purity and sensitivity when used for isolation and purification or analysis. It works very well for purification of modified primers.

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