Oligo Modification Options

We offer a wide variety of oligo modifications such as common and uncommon linkers, spacers, dyes, and modified bases. High performance liquid chromatography (HPLC) and mass spectrometry (MS) are used to control the purity and quality of the synthesised products.

  • 5' end modifcation

    5' FAM

    5' Biotin

    5' P04

    ...

  • Internal modifcation

    FAM-dT

    BHQ-dT

    Biotin-dT

    Digoxin-dT

    ...

  • Nucleotide modification

    2-0-Methyl-A/G/C/U

    5-Me-dC

    2-F -A/G/C/U

    ...

  • 3' end modifcation

    3 TAMRA

    3' Biotin

    3'BHO1

    ...

Modification Group Information

Dual labeled fluorescent probe

5'-FAM, 3'-BHQ1
5'-HEX, 3'-BHQ1
5'-FAM, 3'-BHQ2
5'-HEX, 3'-BHQ2
5'-FAM, 3'-Dabcyl
5'-HEX, 3'-Eclipse
5'-FAM, 3'-Eclipse
5'-HEX, 3'-TAMRA
5'-FAM, 3'-TAMRA
5'-HEX, 3'-MGB
5'-FAM, 3'-MGB
5'-VIC, 3'-BHQ1
5'-TET, 3'-BHQ1
5'-VIC, 3'-BHQ2
5'-TET, 3'-BHQ2
5'-VIC, 3'-MGB
5'-TET, 3'- TAMRA
5'-TAMRA, 3'-BHQ2
5'-TET, 3'-MGB
5'-TAMRA, 3'-MGB
5'-JOE, 3'-BHQ1
5'-CY3, 3'-BHQ2
5'-JOE, 3'-BHQ2
5'-CY3, 3'-MGB
5'-JOE, 3'- TAMRA
5'-ROX, 3'-BHQ2
5'-JOE, 3'-MGB
5'-ROX, 3'-MGB
5'-CY5, 3'-BHQ2
5'-CY5, 3'-BHQ3
5'-CY5, 3'-MGB

Single labeled fluorescein

5'-FAM (FITC)
5'-TET
5'-JOE
5'-HEX
5'-VIC
5'-TAMRA
5'-Су3
3'-Суз
5'-ROX
5'-Cy5
3'-Dabcyl
3'-BHQ-1
3'-BHQ-2
3'-BHQ3
3'eclipse
3'-MGB

Commonly used functional group markers

5'-NH2 C6
3'-NH2 C7
5'-NH2 C12
Spacer C3
Spacer C6
Spacer 9
Spacer C12
Spacer 18
dSpacer
5'-P04
3'-Р04
5'-SH
3'-SH
5'-Digoxin
3'-Digoxin
5'-Biotin
3'-Biotin
3'-Cholesterol

Rare bases

di
dU
2-0-Methyl-A/G/C/U
3'-2,3-ddC
5-Me-dC
2-F-A/G/C/U
LNA
Phosphorothioates

NOTE:
1. Modification primers were used for product purification by HPLC;
2. The synthesis specification can be selected from 20D, 50D, more synthesis amount demand is welcome to consult;
3. The synthesis price of modified primers is calculated as follows: common primer price + modified group price;
4. If the synthesised primers contain special modifying groups, we need to consult and communicate with you in advance.

CASE 1

M-probe

Sequence: ACTCC*****************ATCT
Num. of bases: 26
MW: 8935.14
Purification method: HPLC
Modifications: 5-FAM; 3-BHQ1

CASE II

SSDNA

Sequence: CTC*********АСТС
Num. of bases: 16
MW: 5144.6
Purification method: HPLC
Modifications: 5-Biotin

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