HS Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase produced by mixing Taq antibody with Taq DNA polymerase in optimum proportion. Based on the thermostable properties of Taq antibody, the activity of HS Hotstart Taq DNA Polymerase remains strictly closed at 55°C, minimizing non-specific amplification during the mixing and heating phases of the reaction system. When the reaction is maintained above 30 sec at 95°C, Taq antibody is completely inactivated and the polymerase activity is fully released, ensuring the extremely high amplification sensitivity and specificity of the PCR system. The activation of HS Hotstart Taq DNA Polymerase is not affected by buffer pH, ion strength or other factors. It is suitable for various hot-start PCR and qPCR reactions based on Taq DNA polymerase. It can be used to amplify low-copy genes from complicated templates (genome, cDNA) and is the preferred hot-start Taq DNA polymerase for molecular diagnostic reagents based on PCR/qPCR. The elongation rate is 1kb/min. The PCR product has 3'-d A ends and can be cloned to T vector.
Application
High-specificity amplification & multiplex PCR
High fidelity PCR
Gene expression analysis
Complex template low-copy gene amplification
Features
High specificity, hotstart DNA polymerase with antibody modification, 1kb/min, generates 3'-dA overhangs
